Hybridoma Sequencing

Release time: 2024-11-05 View volume: 97

Case Study

Figure 1: Schematic of Antibody Coding Gene Structure (including 5’ and 3’ untranslated regions)

Figure 2: PCR Amplification of Heavy and Light Chain Variable Regions

Figure 3: Colony PCR Validation Gel

M: DL10000 DNA Marker; 1% Agarose Gel

Sequence	V-GENE and Allele	Functionality	V-REGION Identity (%)	J-GENE and Allele	D-GENE and Allele	AA Junction	Junction Frame
VH	Musmus IGHV1-71-16*01 F	Productive	96.53%	Musmus IGHJ4*01 F	Musmus IGHD2-4*01 F	CAITTGYAMDYW	In-frame
VL	Musmus IGKV4-74*01 F	Productive	97.51%	Musmus IGKJ4*01 F	-	CHQYYRSPFTF	In-frame

Figure 4: Antibody Heavy and Light Chain Sequence Analysis

Figure 5: Antibody Heavy and Light Chain Sequencing Peak Map

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