AtaGenix Laboratories

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Rapid Hybridoma

AtaGenix's Rapid Hybridoma platform delivers validated monoclonal antibodies in as few as 59 days from immunization to frozen hybridoma clones. Our proprietary electrofusion technology ensures high fusion efficiency and rapid screening, giving you stable, renewable antibody sources for research, diagnostics, and therapeutic development.

With over 15 years of hybridoma experience and a >90% project success rate (per pre-agreed acceptance criteria), AtaGenix is trusted by global pharma, biotech, and academic partners for high-quality custom monoclonal antibody generation.

Our milestone-based project governance ensures full transparency: you approve at each checkpoint before moving forward. All deliverables include frozen hybridoma cells (≥5 vials), purified antibody (1–2 mg), ELISA validation data, isotype report, and Certificate of Analysis (COA).

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Two Service Tiers, One Commitment to Quality

Whether you need a complete antibody development campaign or sequencing of existing hybridoma lines, AtaGenix delivers with precision and speed.

Standard Hybridoma Package

Timeline: 59 days | Mouse & Rat Hosts

Complete turnkey service from antigen to validated hybridoma clones:

 Customized immunization protocol (mouse/rat)  Electrofusion & ELISA screening
 Subcloning to monoclonality  Isotype determination
 Frozen cell stocks (≥5 vials)  Purified antibody (1–2 mg) + COA

Premium Hybridoma Package

Everything in Standard, plus advanced characterization

 Full VH/VL sequencing  Western blot & IHC validation
 Epitope mapping (optional)  Ascites or recombinant production
 Extended cell banking  SPR/BLI affinity measurement (optional)

Hybridoma Sequencing & Recombinant Expression

Already have hybridoma lines? We offer high-accuracy variable region and full-length sequencing with recombinant expression using our proprietary pATX3.0 vector.

 Variable region + full-length sequencing  CDR region analysis report
 Recombinant expression: 200–500 mg/L yield  Turnaround: 2 weeks
 Accepts cultured cells or frozen cell lines

When to Choose Hybridoma

Your Need Why Hybridoma
Long-term renewable supply Frozen cell stocks provide indefinite antibody production with consistent lot-to-lot performance
Matched antibody pairs Large screening panels naturally identify capture/detection pairs for sandwich ELISA and lateral flow
Diagnostic development Full-length IgG with native Fc function, ideal for immunoassay and IVD applications
Budget-sensitive projects Most cost-effective route to validated monoclonal antibodies for standard immunogenic targets

Quality Specifications

59

Days to Delivery

>95%

Purity (SEC-HPLC)

10-7–10-9

Affinity KD (M)

100%

Full IP Ownership

Timeline counted from sample receipt. Actual duration depends on antigen availability and immune response. Milestone-based delivery with pre-agreed acceptance criteria. Quote-based pricing.

Hybridoma Development Workflow

Our streamlined 59-day process moves through four clearly defined milestones with client sign-off at each gate.

01

Immunization

Week 1–4

Customized protocol
Mouse or rat hosts
Flexible antigen formats

02

Fusion & Screening

Week 5–6

Electrofusion platform
ELISA-based screening
Hit identification

03

Subcloning & QC

Week 6–8

Subcloning to monoclonality
Isotype determination
Stability confirmation

04

Delivery

Week 8–9

Frozen hybridoma cells
Purified antibody (1–2 mg)
Validation data + COA

Milestone Deliverables

Milestone Timeline Deliverables Client Gate
M1: Immunization Week 1–4 Serum titer check (ELISA); immunization progress report ✓ Approve
M2: Fusion & Hits Week 5–6 Primary ELISA hits; clone count; shortlist for subcloning ✓ Select
M3: Subcloning Week 6–8 Monoclonal clones confirmed; isotype report; supernatant samples ✓ Confirm
M4: Delivery Week 8–9 Frozen cells (≥5 vials) + purified Ab (1–2 mg) + ELISA data + COA + final report ★ Complete

Not sure if hybridoma is the right fit? Compare it with Single B Cell (45 days) and Phage Display (VHH/Nanobody) to find the best match for your target and application.

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