AtaGenix Laboratories

Home - Antibody Discovery

Rapid Hybridoma

Hybridoma antibody technology produces monoclonal antibodies but faces challenges like instability and the need for liquid nitrogen preservation. Hybridoma sequencing enables recombinant expression, ensuring stability and preventing biological contamination.

AtaGenix offers high-accuracy hybridoma sequencing and recombinant antibody expression, achieving 200-500 mg/L yields in 2 weeks with the proprietary pATX3.0 vector.

 

 

Contact Us

At AtaGenix, we pride ourselves on over 10 years of expertise in delivering custom hybridoma monoclonal antibody services. Our process is designed to meet the highest standards of quality and efficiency, ensuring your research and therapeutic projects are supported by the best monoclonal antibodies available.

Antigen Design

Antigen Design

  • Define immunization strategy
  • Perform peptide synthesis, gene synthesis, and protein production
  • Opt for DNA immunization
 
Immunization

Immunization

  • Immunize 5 mice using a proprietary protocol
  • Generate a robust immune response
 
Cell Fusion

Cell Fusion

  • Collect splenocytes from 2 mice
  • Perform cell fusion with a myeloma cell line
  • Create hybridomas
 
Hybridoma Selection

Hybridoma Selection and Screening

  • Select hybrid cells using HAT selection
  • Expand and screen monoclones via ELISA
  • Target highest-quality antibodies
 
Monoclone Selection

Isolation and Selection of the Best Monoclones

  • Isolate monoclones through limiting dilution
  • Expand and screen monoclones by ELISA
  • Select for highest-quality antibodies

More Featured Antibody Services

Featured Services

Ask us