AtaGenix Laboratories

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Phage Display

Struggling with monoclonal antibody development? AtaGenix offers phage display custom services to streamline the process and deliver high-quality antibodies in less than 2 months. Our solutions address challenges like immunogenicity and complex humanization by providing direct access to human monoclonal antibodies. With the world’s first immune phage display library derived from human cancer patient donors and diverse naïve libraries featuring Fab, scFv, and VHH formats, we ensure unmatched diversity and efficiency.

AtaGenix guarantees at least three high-affinity binders tailored for applications such as ELISA, WB, or Flow Cytometry. Backed by extensive expertise and a proven track record, we are leaders in therapeutic, research, and diagnostic antibody development. Partner with us for innovative solutions and accelerated project success.

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Phage Display Technology Roadmap

A streamlined antibody development workflow, from immune library construction to functional validation. Designed for high diversity, precise screening, and rigorous testing, ensuring stable and effective antibody candidates for research, therapy, or diagnostics.

Step 1: Immune Library Construction

  • Isolation of relevant immune cells (e.g., PBMC or B cells)
  • Construction of an scFv, Fab, or other antibody format library

Milestone: Library diversity > 10⁹

Step 2: Antibody Screening

  • Enrichment via phage display or other display technologies
  • Identification of high-affinity binders using specific targets
  • Competitive ELISA or equivalent assays

Milestone: Selection of cross-reactive binders

Step 3: Recombinant Expression

  • Expression of selected binders in mammalian or bacterial systems
  • Characterization of binder bioactivity and stability

Milestone: Stable and active antibody candidates

Step 4: Functional Validation

  • Screening for neutralizing or binding activity
  • Validation through in vitro and in vivo assays

Milestone: Identification of lead candidates

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