Mammalian Cell Protein Expression Services — HEK293 & CHO Platform

High Expression Efficiency

Optimized expression systems scalable from mL to 100 L transient and up to 200 L+ serum-free suspension batches. In-house high-yield protocols increase output 3–6× over standard methods.

Native Post-Translational Modifications

Mammalian cell systems preserve glycosylation, phosphorylation, and disulfide bonding, ensuring biological activity consistent with native proteins.

Flexible Customization

Multiple expression vectors, tag options (His, Fc, GST, FLAG), and purification protocols tailored to target protein characteristics and downstream application requirements.

1

Project Evaluation

Custom expression strategy & feasibility analysis based on target protein information.

2

Gene Cloning & Vector

Gene optimization, cloning into optimized vectors, condition fine-tuning.

3

Transfection & Expression

Advanced transfection into HEK293 or CHO cells with optimized high-yield conditions.

4

Purification & QC

Multi-step purification, SDS-PAGE, SEC-HPLC, endotoxin testing (<0.1 EU/mL).