Mammalian HEK293 Expression

The experimental results demonstrate that the SARS-CoV-2 S protein expressed in mammalian cells achieved a high yield during the purification process. Clear target protein bands were observed in the elution fractions (E1-E8) with minimal impurities, indicating effective purification. This expression and purification workflow produces high-purity S protein, facilitating the development of diagnostic reagents.

Lane Descriptions:
Lane MW: Protein Marker
Lane IN: Input
Lane FT: Flow-through
Lane W1, W2: Wash fractions
Lane E1-E12: Elution fractions