MTiterFast® Adjuvant – High-Performance Aqueous Adjuvant for Mice

Product Description

MTiterFast^® Adjuvant is a pH-neutral aqueous adjuvant with a bluish transparent appearance. The primary component is a high-molecular-weight polymer, whose hydrophobic ends bind to protein antigens (including peptides or small molecules conjugated to carrier proteins) without altering their structure. Once injected into the animal, the adjuvant slowly releases the bound antigen, gradually stimulating the immune system. All auxiliary materials meet human-use pharmaceutical standards, ensuring that the adjuvant itself is non-toxic, non-immunogenic, and highly biocompatible. The adjuvant significantly reduces adverse reactions, such as inflammation or injection site necrosis, compared to Freund’s adjuvant. Additionally, MTiterFast^® contains no protein components, preventing antibody production against the adjuvant itself. By reducing antigen consumption and shortening the immunization cycle, it effectively lowers antibody production costs. MTiterFast^® is primarily suitable for use in mice.

MTiterFast^® Adjuvant has been tested in 1704 BALB/c mice with various antigen types, including recombinant proteins, small molecules, and peptides. It is ideal for developing polyclonal antibodies and hybridomas in mice. Over 300 high-quality antibodies have been successfully developed using MTiterFast^®, with applications in WB, IHC, IP, IF, Sandwich ELISA, indirect ELISA, Neutralization assays, and more. Below is a summary of serum titer statistics.

Fig 1.MTiterFast Aqueous Adjuvant for Mice

The graph shows the serum titer distribution of 1704 BALB/c mice immunized three times with MTiterFast^®. Approximately 60% of the antigens used were recombinant proteins, 35% were peptides, and 5% were small molecules. Cutoff OD = 0.25.

Usage Recommendations

Immunization Protocol: Three immunizations are recommended (Day 0, Day 7, Day 14). Serum can be collected on Day 21 for titer evaluation. If titers are lower than expected, administer a booster on Day 21 or 22.

Antigen Application and Recommended Dosage:

1) For protein antigens, inactivated whole viruses, or virus-like particles (VLPs): Use 40-60 μg per mouse per dose, diluting the antigen to 100 μL and mixing with 50 μL of MTiterFast^® before injection.

2) For small molecules or peptides conjugated to carrier proteins: Use 80-120 μg per mouse per dose, diluting the antigen to 100 μL and mixing with 50 μL of MTiterFast^® before injection.

Injection Methods: Inject 150 μL of the antigen-adjuvant mixture per mouse. Common injection methods include subcutaneous multi-point injections, intraperitoneal injections, and intramuscular injections. The recommended method is two subcutaneous injections at the dorsal neck and one intraperitoneal injection, dividing the mixture equally among the three sites.

Preparation Method: Dilute the antigen with sterile saline or PBS to the recommended volume based on the antigen usage and the planned number of animals. Mix with the required amount of adjuvant and inject immediately after thorough mixing.

Storage Conditions: Store at 4-8°C. Avoid freezing. Shelf life: Two years.

Precautions

1) Precipitation after mixing with antigen is normal. Mix thoroughly before loading into the syringe and inject immediately after loading.

2) The adjuvant is optimized for use in mice and may have slightly reduced efficacy in other species.

3) Handle with care when opening to avoid injury from the aluminum seal.