AtaGenix Laboratories
AtaGenix Laboratories
Release time: 2025-03-11 View volume: 802
Frequently asked questions about polyclonal antibody production: antigen types, immunization schedules, purification strategies, and titer measurement — covering practical guidance for optimizing specificity, yield, and downstream application performance.
AtaGenix produces polyclonal antibodies using a wide range of antigen types, including proteins, peptides, DNA, small molecules, and cell lysates. Custom antigen production is available, or clients may provide their own antigens for immunization.
Maximizing antibody specificity and immune response depends on selecting the right antigen format:
Proteins: The most common choice, ensuring broad epitope exposure for multi-application antibodies.
Peptides: Custom-synthesized for linear epitopes, ideal for Western Blot and ELISA where a defined epitope is required.
DNA: Used in special cases where traditional protein/peptide immunization is difficult (e.g., membrane proteins).
Small Molecules: Requires conjugation with carrier proteins (KLH, BSA) to elicit an immune response.
51 days
Protein-based pAb schedule
70 days
Peptide-based pAb schedule
Extended immunization schedules are available for higher titers. Lower antigen doses with longer immunization periods generally yield the best results in terms of affinity maturation.
After reaching peak antibody titers, AtaGenix purifies polyclonal antibodies using two primary strategies:
Protein A/G Purification: High-yield IgG capture with minimal non-specific binding. Suitable for most downstream applications.
Antigen-Specific Affinity Purification: Removes non-specific antibodies for maximum target specificity. Recommended for IHC, IF, and applications requiring low background.
Serum samples from hyperimmunized hosts are regularly tested by ELISA to monitor titer progression. Western Blot validation is available upon request for additional confirmation of antibody specificity and reactivity against the target antigen.
Have questions about antigen design, immunization strategy, or purification options for your polyclonal antibody project? AtaGenix provides end-to-end custom pAb development across multiple host species.
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