Preparation of MHC-Peptide Complex Proteins

Release time: 2024-11-05 View volume: 67

Expression of HLA-I Protein in E. coli - Peptide Complex

HLA-I Protein Expression and Complex Formation

After expressing and purifying HLA protein and β2M protein in E. coli, protein refolding experiments were conducted to produce stable HLA/β2M/peptide complexes (pMHC). By adding streptavidin (SA), stable tetramers were successfully formed.

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Preparation of MHC-Peptide Complex Proteins

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