Product Description
The BSA ELISA Kit is a high-sensitivity assay designed for the quantitative detection of bovine serum albumin (BSA) in biological samples. This double-antibody sandwich ELISA employs monoclonal anti-BSA antibodies as coating and detection antibodies. The kit includes reference standards traceable to the US National Institute of Standards, enabling detection limits as low as 1 ng/mL.
Experimental Overview
- Catalog No: ATK00051
- Detection wavelength: 450 nm
- Sensitivity: 1 ng/mL
- Storage conditions: 2-8°C, shelf life of 6 months
Components & Storage
| Component | Details | Storage |
|---|---|---|
| 96-well coated plate | Monoclonal anti-BSA antibody-coated strips | 2-8°C |
| BSA Standards | 32, 16, 8, 4, 2, 1, 0 ng/mL (0.4mL each) | 2-8°C |
| Internal Reference | 20 ng/mL BSA, 1x0.4mL | 2-8°C |
| TMB Substrate | 6.5mL each (A & B) | 2-8°C |
| Stop Solution | 2N H2SO4, 13mL | 2-8°C |
| Sample Diluent | 13mL | 2-8°C |
| 10x Wash Buffer | 50mL | 2-8°C |
| HRP-labeled anti-BSA antibody | 0.13mL | 2-8°C |
| HRP Antibody Diluent | 13mL | 2-8°C |
Procedure Summary
- Prepare reagents and standards as per instructions.
- Add 50 μL standards and samples to the corresponding wells in duplicate.
- Add 100 μL diluted HRP-conjugated antibody to each well and incubate at 20-24°C for 1 hour.
- Wash the plate 4 times with diluted wash buffer and blot dry.
- Add 100 μL TMB substrate to each well, incubate at 20-24°C for 10 minutes in the dark.
- Add 100 μL stop solution to each well, mix gently for 5 seconds.
- Read absorbance at 450 nm using a microplate reader.
Storage & Notes
- Store all reagents as instructed in the kit manual.
- Ensure that all pipettes, tips, and containers are free from BSA contamination.
- Handle stop solution with caution as it is corrosive.
- Unused strips should be stored in a resealable bag with a desiccant at 4°C.
