Xten™ Mab Single B Rabbit Monoclonal Antibody Development Case Study

Utilizing rabbit single B-cell antibody technology, an Anti-human CD86 rabbit monoclonal antibody was developed. Rabbits with serum titers greater than 128K were selected, and PBMCs were isolated for flow sorting to obtain human CD86 antigen-specific B-cell plates.

Fig 1. Flow Sorting Results of Rabbit Single B-Cells

The XtenCHO high-density transient expression system by AtaGenix was used for recombinant expression of positive clones. Purified antibodies were then validated for functionality.

Fig 2. Analysis of Recombinant Expression of Rabbit Monoclonal Antibodies

Purified antibodies were further validated via IF and endogenous WB assays. IF results showed high sensitivity and precise antigen localization, while WB analysis revealed a clear primary band with no nonspecific binding.

Fig 3. IF Analysis of Anti-human CD86 Rabbit Monoclonal Antibody

RAJI cells, primary antibody 1:50, secondary antibody 1:50, exposure time 30ms

Fig 4. Endogenous WB Analysis of Anti-human CD86 Rabbit Monoclonal Antibody

Lane 1: Daudi cell lysate; Lane 2: Raji cell lysate.