Prokaryotic Protein Expression Services — E. coli 3H Platform

>95% Expression Success

Optimized vectors (pET, pGEX series) with specialized strains — T7E for high expression, C41 for toxic proteins, Arctic for low-temp solubility — address difficult-to-express targets.

Cost-Effective & Scalable

15 shakers, 12 ultrasonic disruptors, pilot-scale homogenizers. Short culture cycles, simple operation, and low media costs from mg research-grade to gram-scale production.

Diverse Protein Types

Antigens, enzymes, tagged fusion proteins (His, GST, MBP, SUMO), and antibody immunogens. Soluble expression, inclusion body refolding, and on-column refolding strategies available.

1

Consultation & Evaluation

Custom expression strategy based on target protein properties (solubility, toxicity, modification needs).

2

Vector & Strain Screening

Gene optimization, cloning into selected vector, host strain selection, and sequence verification.

3

Expression & Purification

Induction optimization, IPTG/auto-induction, multi-step purification (affinity, IEX, SEC).

4

QC & Delivery

SDS-PAGE, endotoxin testing (<0.1 EU/mL), activity validation. Protein samples + full report.