普健生物（武汉）科技有限公司（AtaGenix）
Recombinant Human LAG3 protein ,C- His Tag
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- 产品信息（Product Details）
- 前沿进展（Frontier progress）
- 如何订购
- 说明书
概述（Summary）icon
英文全称
Recombinant Human LAG3 protein ,C- His Tag
纯度（Purity）
>90% as determined by SDS-PAGE
内毒素（Endotoxin level）
Please contact with the lab for this information.
蛋白构建（Construction）
A DNA sequence encoding the human LAG3(Met1-Leu450) was fused with the C-terminal His Tag
Accession #
P18627
表达宿主（Host）
Mammalian cells
种属（Species）
Homo sapiens (Human)
预测分子量（Predicted Molecular Mass）
49.61kDa
制剂（Formulation）
Supplied as solution form in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl or lyophilized from 50 mM Tris-HCl, pH 7.5, 150 mM NaCl.
运输方式（Shipping）
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性&储存（Stability &Storage）
Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.
复溶（Reconstitution）
Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
电泳图（SDS-PAGE image）icon
背景（Background）icon
背景介绍
Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. LAG3 /CD223 involved in lymphocyte activation. LAG3 /CD223 binds to HLA class-II antigens.
分子别名（Alternative Names）
LAG3,CD223,FDC
参考文献（References）
Chen, Yu, Liu, Wu, Qin, Zhou (2020) [Preparation of anti-lymphocyte activation gene 3 (LAG-3) fully human antibodies] Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 36(5) 451-456
Noteicon
For research use only .
Objective Lymphocyte activation gene 3 (LAG-3) is a potential therapeutic target of tumor. This work aims to screen for anti-LAG-3 human scFvs from a human phage antibody library, and transform them into fully human antibodies. Methods The human phage antibody library was screened for anti-LAG-3 human scFvs. The specificity and affinity of phage scFvs were determined by ELISA and surface plasmon resonance (SPR), respectively. The anti-LAG-3 full human antibodies were obtained using eukaryotic expression system. Results Through 3 rounds of screening, we obtained 4 phage scFv clones that could bind to human LAG-3 recombinant protein. Affinity determination showed that the highest affinity reached 3.48×10-10. Then through the construction of whole human antibody expression vector, expression in eukaryotic cells, and purification, 3 clones of anti-LAG-3 full human antibodies were harvested finally. Conclusion Using a large-capacity human antibody phage display library, we obtained three anti-LAG-3 full human antibodies with high affinity.
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