Recombinant Human CD257/BAFF/BLyS/TNFSF13B protein ,C- His Tag

Recombinant Human CD257/BAFF/BLyS/TNFSF13B protein ,C- His Tag

>90% as determined by SDS-PAGE

Please contact with the lab for this information.

A DNA sequence encoding the human TNFSF13B(Ala134~Leu285 ) was fused with the C-terminal His Tag

Q9Y275

Mammalian cells

Homo sapiens (Human)

17.97kDa

Supplied as solution form in 50 mM Tris-HCl, pH 8.0, 150 mM NaCl or lyophilized from 50 mM Tris-HCl, pH 8.0, 150 mM NaCl.

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.

Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.

B-cell activating factor (BAFF) is also known as tumor necrosis factor ligand superfamily member 13B , TNFSF13B, BAFF, B Lymphocyte Stimulator (BLyS) , cluster of differentiation 257 (CD257), DTL, TNF- and APOL-related leukocyte expressed ligand (TALL-1), THANK, TNFSF20, ZTNF4, and is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. It is expressed as transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R, TACI, and BCMA, all of which have differing binding affinities for it. These receptors are expressed mainly on mature B lymphocytes (TACI is also found on a subset of T-cells and BCMA on plasma cells). TACI binds worst since its affinity is higher for a protein similar to BAFF, called a proliferation-inducing ligand (APRIL). BCMA displays an intermediate binding phenotype and will work with either BAFF or APRIL to varying degrees. Signaling through BAFF-R and BCMA stimulates B lymphocytes to undergo proliferation and to counter apoptosis. All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors, although BAFF has been known to be active as either a hetero- or homotrimer. BAFF acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells.

TNFSF13B,BAFF,BLYS,CD257,DTL,TALL-1,TALL1,THANK,TNFSF20,ZTNF4

Talbot, Saada, Barthout, Gallet, Gachard, Abraham, Jaccard, Troutaud, Lalloué, Naves, Fauchais, Jauberteau (2020) BDNF belongs to the nurse-like cell secretome and supports survival of B chronic lymphocytic leukemia cells Scientific reports 10(1) 12572

For research use only .

Evading apoptosis and sustained survival signaling pathways are two central hallmarks of B-cell chronic lymphocytic leukemia (B-CLL) cells. In this regard, nurse-like cells (NLC), the monocyte-derived type 2 macrophages, deliver stimulatory signals via B-cell activating factor (BAFF), a proliferation-inducing ligand (APRIL), and the C-X-C Motif Chemokine Ligand 12 (CXCL12). Previously, we demonstrated that brain-derived neurotrophic factor (BDNF) protects B-CLL cells from spontaneous apoptosis by activating the oncogenic complex NTSR2-TrkB (neurotensin receptor 2-tropomyosin-related kinase receptor B), only overexpressed in B-CLL cells, inducing anti-apoptotic protein Bcl-2 (B-cell lymphoma 2) expression and Src kinase survival signaling pathways. Herein, we demonstrate that BDNF belongs to the NLC secretome and promotes B-CLL survival. This was demonstrated in primary B-CLL co-cultured with their autologous NLC, compared to B-CLL cells cultured alone. Inhibition of BDNF in co-cultures, enhances B-CLL apoptosis, whereas its exogenous recombinant activates pro-survival pathways in B-CLL cultured alone (i.e. Src activation and Bcl-2 expression), at a higher level than those obtained by the exogenous recombinant cytokines BAFF, APRIL and CXCL12, the known pro-survival cytokines secreted by NLC. Together, these results showed that BDNF release from NLC trigger B-CLL survival. Blocking BDNF would support research strategies against pro-survival cytokines to limit sustained B-CLL cell survival.

1搜索商品：关键字搜索；分类导航搜索；产品联系：手机/微信同号：15972188750，座机：027-65279366（推荐 ！）

2产品选购：确定好产品信息与规格，向产品客服询问底价

3提交订单：向客服提交收货人信息，选择送货方式，支付方式，发票信息

4产品发货：发票随产品一起发送，耐心等待收货即可

质量保证：

所售产品均经过严格的质量检验，确保所供产品的技术指标与生产厂家的目录、说明书、质检报告上的描述一致，请放心选购。

质量与免责说明：

买方验收产品时，如发现产品短缺、不匹配、包装破损等情况，请保持产品原包装状态，并在收货七个工作日内，以书面方式向我司提出异议。逾期未提出异议视为合格。

买方保管产品时，应按照产品标签、说明书所示保管条件保存，如系保管不当导致产品质量问题，恕不受理。

买方使用产品时。如发现产品质量问题，应在使用初期提出，而非在使用完或快使用完时提出，以备我司回收产品，确认产品质量。如确属质量问题，我司负责调换或退货。发生索赔事宜，我司将在产品价格额度范围内酌情赔偿，恕不受理超出产品本身价值之部分。自收货之日起，超过三个月未反映产品问题，恕不退换。

我司所提供的产品仅限于专业人士的科学研究使用，不得用于临床诊断或者治疗，若有单位或者个人擅自改变我司产品用途，我司不承担任何责任。